Fig. 6: SUV39H1 negatively regulated p47phox and gp91phox expression.
From: Aging increases vulnerability to stress-induced depression via upregulation of NADPH oxidase in mice
a Experimental design. siSUV39H1 or siCON was stereotaxically injected in the CA3 region (red arrows). Blue arrow, tissue preparation point. b Expression levels of SUV39H1, p47phox, gp91phox, and p67phox transcripts in the CA3 region (n = 8, each). c Photomicrographs showing DHE-stained CA3 region of mice injected with siSUV39H1 or siCON. Scale bars, 200 µm. d Quantification of DHE-reactive ROS levels (n = 7 mice/group). e Diagram showing the promoter region of the p47phox. f–i ChIP-qPCR analysis showing the levels of SUV39H1, trimeH3K9, dimeH3K9, and acH3K9 binding to the promoter of the p47phox in the hippocampus of mice at 2 and 18 months of age. P1 and P2, the promoter regions used for ChIP-qPCR analysis. j Diagram showing the promoter region of the gp91phox. k–n ChIP-qPCR analysis showing the levels of SUV39H1, trimeH3K9, dimeH3K9, and acH3K9 binding to the promoter of the gp91phox in the hippocampus of mice at 2 and 18 months of age. P1 and P2, the promoter regions used for ChIP-qPCR analysis (p47phox, SUV39H1 n = 6, trimeH3K9, n = 5, dimeH3K9, n = 4, and acH3K9, n = 5; gp91phox, SUV39H1 n = 6, TrimeH3K9, n = 4, DimeH3K9, n = 7, and acH3K9, n = 4). *p < 0.05 and **p < 0.01.
