Fig. 7: RA blocked GC-induced changes in the Ppp2ca-SUV39H1-p47phox pathway and stress-induced depression-like behavior in aged mice.
From: Aging increases vulnerability to stress-induced depression via upregulation of NADPH oxidase in mice
a–d Ppp2ca, SUV39H1, and p47phox transcript levels in HT22 cells treated with GC GC + RA, or RA alone. RA, 50 or 100 µM (n = 6, each; a One-way ANOVA, F(2,15)=8.933, p = 0.0028; b One-way ANOVA, F(2,15)=27.62, p < 0.0001; c One-way ANOVA, F(2,15)=13.22, p = 0.0005; d One-way ANOVA, F(2,15)=12.11, p = 0.0007). e, f Photomicrographs showing DHE-reactive ROS levels in HT22 cells treated with GC, and GC plus RA, and quantification levels (n = 5, each; One-way ANOVA, F(2,12)=5.31, p = 0.0223). g Experimental design for treatment with daily 2-h restraint for 5 days (RST5d) or RST5d plus RA in aged (18M) mice, and following behavioral tests. Arrow, time point for tissue preparation. h–k Expression levels of Ppp2ca, SUV39H1, p47phox, and gp91phox transcripts in the hippocampus of indicated groups (n = 6, each; Ppp2ca, One-way ANOVA, F(2,15)=4.458, p = 0.0302; SUV39H1, One-way ANOVA, F(2,15)=15.42, p = 0.0002; p47phox, One-way ANOVA, F(2,15)=15.83, p = 0.0002; gp91phox, One-way ANOVA, F(2,15)=4.249, p = 0.0345). l Representative tracking plots and time spent in the target and non-target fields (n = 7 mice/group; target field, One-way ANOVA, F(2,18)=8.003, p = 0.0033; non-target field, One-way ANOVA, F(2,18)=7.969, p = 0.0033). m Sociability index in the sociability test (n = 7 mice/group; One-way ANOVA, F(2,18)=7.983, p = 0.0033). n Immobility time in the FST (n = 7 mice/group; One-way ANOVA, F(2,18)=13.22, p = 0.0033). o, p K-Means clustering of individuals in the sociability x FST matrix plotting with z-scores and % composition of each group in the clusters (cluster1, 100% for CON, 14.3% for RST5d, and 85.7% for RST5d + RA; cluster2, 0% for CON, 85.7% for RST5d, and 14.3% for RST5d + RA). *p < 0.05 and **p < 0.01. One-way ANOVA followed by a Newman–Keuls post hoc test.
