Fig. 1: Protocol of AUC-SAS for multi-component system. | Communications Biology

Fig. 1: Protocol of AUC-SAS for multi-component system.

From: Integral approach to biomacromolecular structure by analytical-ultracentrifugation and small-angle scattering

Fig. 1

For aggregation system, in Steps 3 and 4, the contribution of monomer in the scattering intensity is estimated because that of aggregation is not measurable directly. On the other hand, if all ij(q) (j ≠ k) are obtained by individual measurements, such as an association–dissociation equilibrium system, Steps 3 and 4 are skipped.

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