Fig. 4: Metastatic potential of breast PDXs.

a Bar plots of fraction of animals with gross metastatic lesions at various anatomical sites at routine necropsy (median n = 11, range n = 5–47). ALN axillary lymph node. b Representative H&E images of lung metastatic lesions. Scale bar 400 μm, inset scale bar 50 μm. Complete lung micrometastasis H&E images are in Supplementary Fig. 7a. c Kaplan–Meier of progression-free survival (PFS) for patients whose PDXs developed frequent metastases (top 20%) in vivo versus those exhibiting less frequent or no metastases. d Schematic of metastatic lesions and timing since diagnosis for GCRC1986 patient. Squared sample is the lesion used to generate the PDX. e Bar chart of time of resection (at 10 mm) and endpoint for each PDX animal (n = 9). Bars represent individual mice. f Piecharts for frequency of metastasis observed to ALN, lung, liver, abdomen and brain. g Representative H&E images of metastatic lesions to specific anatomic sites. The same formatting applies to h–k for GCRC1991 and l–n for GCRC1971. o Gross picture of skull-base metastasis in PDX (left), with corresponding H&E image (middle). p Gadolinium MRI of head of the patient GCRC1971 shows 1.1 cm cavernous sinus mass (arrowhead).