Fig. 3: FeS cluster loss affects DNA2’s ability to grip shorter DNA substrates.
From: The iron–sulphur cluster in human DNA2 is required for all biochemical activities of DNA2
a Representative EMSA of wild-type DNA2/DNA complexes using 5′ overhang substrates containing ssDNA of different lengths. Final protein concentration is 20 nM, final substrate concentration 4 nM. b Representative EMSA of DNA2 C396S/DNA complexes using 5′ overhang substrates containing ssDNA of different lengths. Final protein concentration is 200 nM, final substrate concentration 4 nM. c Quantification of DNA binding from a and b, expressed in % (n = 3 independent experiments; error bars, SD).
