Fig. 2: Histone acetylation and deacetylation correspond to epigenetic-mediated phenotype changes in melanoma.
From: Geometric regulation of histone state directs melanoma reprogramming
a Representative immunofluorescence images of histone acetylation and deacetylation for B16F0 cells cultured on a panel of shapes. b Flow cytometry characterization of histone acetylation and deacetylation in B16F0 cells and quantification of the difference between patterned and non-patterned cells for each condition with thresholds (green dots) assigned by overlapping regions of patterned and non-patterned plots. c Single cell analysis of histone acetylation and deacetylation in B16F0 cells cultured in two different regions of the circular shape (n = 3, biological replicates, total 100 cells for each marker). Boxes represent 25th to 75th percentile and whiskers represent minimum-maximum. Horizontal lines and points within boxes represent the median and mean respectively for three duplicates. d Western blots for H3K9ac with non-patterned or spiral-patterned melanoma cells. e Shapes regulating curvature and perimeter/area to explore the relationship between H3K9ac and HDAC3 (n = 3, biological replicates, total 12 patterns for each condition, except for 20 μm-width line shape: 7 patterns and 40 μm-width medium torus shape: 9 patterns). Significant differences were compared to those cultured on 60 μm line shapes. Scale bars, 50 μm. *P < 0.05, ANOVA. Error bars represent s.d.
