Fig. 4: SOX10 and PRDM14 are involved in regulating the epigenetic state associated with the MIC phenotype.
From: Geometric regulation of histone state directs melanoma reprogramming
a ChiP-seq occupancy for H3K9ac over SOX10, intensity ratio of SOX10 normalized by the fluorescent signal to the intensity of DAPI (n = 3, biological replicates, total 20 patterns for each condition), and representative immunofluorescence confocal images of SOX10 for B16F0 cells cultured in a panel of shapes. b Results of qPCR to measure the expression of genes associated with the differential peaks of H3K9ac/SOX10 motif. (n = 4, biological replicates) c Results of qPCR to measure the gene expression of stemness (SOX2, OCT4, Nanog) and MIC (CD271, CD133, Jarid1B) for B16F0 cells cultured on spiral geometries for 5 days with PRDM14 or scrambled siRNAs (n = 4, biological replicates). d Representative immunofluorescence images and relative intensity of representative MIC marker, CD271 for hMela cells cultured for five days on circular or spiral geometries or non-patterned substrates. (n = 3, biological replicates, total 12 patterns for each condition). e Representative immunofluorescence confocal image and relative intensity of PRDM14 expression for B16F0, B16F10, and hMela cells cultured for five days on circular or spiral geometries or non-patterned substrates. (n = 3, biological replicates, total 12 patterns for each condition). Scale bars, 50 μm. *P < 0.05, **P < 0.005, ***P < 0.0005, ANOVA. Error bars represent s.d.
