Fig. 2: Amino acids 460–478 of Rhed are critical for hemin binding and RNA interaction. | Communications Biology

Fig. 2: Amino acids 460–478 of Rhed are critical for hemin binding and RNA interaction.

From: Select amino acids in DGCR8 are essential for the UGU-pri-miRNA interaction and processing

Fig. 2

a, d Purified G478del (a) and G460del (d) proteins analyzed by SDS-PAGE. b, e The EMSAs for G478del (b) and G460del (e). Various amounts of G478del (b) or G460del (e) (ranging from 0 to 13 μM) were mixed with 1 μM of 10L10_UGU or 10L10_noUGU in a 10 μL reaction solution. c, f Quantification of the EMSA data in (b) and (e) for G478del and G460del, respectively. The density of each RNA band on the gel was measured using Image Lab 6.0 (Bio-Rad), and the results were obtained from three independent experiments.

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