Fig. 2: Protein synthesis with iVTtRNAs.
From: Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs
a Genetic code table used for protein synthesis with iVTtRNAs. DNA sequences for protein expression were designed according to this table. b SDS-PAGE analysis of synthesized proteins labeled with [35S]Met. c Fluorescence images of synthesized sfGFP in reaction mixtures. Temperature dependence of the synthesized protein yield. Yields with iVTtRNAs (d) and yields with native tRNA mixtures (e) are shown. All experiments were performed with 60 A260 unit/mL iVTtRNA mixtures or 40 A260 unit/mL native tRNA mixtures. Error bars indicate standard deviation of independent repeats of triplicate measurements. Each dot represents individual observed value. An asterisks indicate that P values < 0.05. Welch’s t test was applied between yields at 30 and 37 °C.
