Fig. 4: Genetic code redesigning with iVTtRNAs.
From: Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs
a Redesigned genetic code table using newly prepared iVTtRNAAlaCGA but without iVTtRNAAlaGGC. The newly assigned UCG codon for Ala is highlighted in red, and original Ala codons not assigned to any amino acids in the redesigned genetic code table are shaded with gray. b SDS-PAGE analysis of synthesized protein labeled with [35S]Met. c Specific activity of synthesized DHFR. All experiments were performed with 40 A260 unit/mL native tRNA mixtures or 20 A260 unit/mL iVTtRNA mixtures and 40 A260 unit/mL IPEN-iVTtRNA mixtures. Error bars indicate standard deviation of independent repeats of triplicate measurements. Each dot represents individual observed value. An asterisk indicates that P value < 0.05. Welch’s t test was applied between DHFR activities synthesized with native tRNA mixtures and iVTtRNA mixtures.
