Fig. 4: Transcriptomic analysis implicates neurogenesis and other pathways in mutant H3.3 gliomagenesis. | Communications Biology

Fig. 4: Transcriptomic analysis implicates neurogenesis and other pathways in mutant H3.3 gliomagenesis.

From: Reciprocal H3.3 gene editing identifies K27M and G34R mechanisms in pediatric glioma including NOTCH signaling

Fig. 4

a Multi-dimensional scaling plot of cell panel gene expression. Biological replicate samples are denoted by .1 and .2 suffixes; label colors reflect H3.3 mutation status: WT (black), G34R (purple), K27M (blue). b Hierarchal heatmap of top 200 differentially expressed genes. c Heatmap of RT-qPCR expression values performed on select neurogenesis and NOTCH pathway genes across our cell panel. Relative log2FC values normalized to respective parental cells are tabulated. Data are the means of n = 3 biologically independent samples; *p < 0.05, **p < 0.01, ns = not significant. d, e Venn diagrams of overlapping genes that are significantly upregulated and downregulated in H3.3K27M mutant cells compared to H3.3WT counterparts, respectively. f Top gene ontology clusters that are enriched across all H3.3K27M cell lines relative to their matched WT cells. g, h Venn diagrams comparing genes that are significantly upregulated and downregulated in H3.3G34R and H3.3K27M cells. i Top gene ontology clusters that are enriched across both H3.3G34R cell lines relative to their matched WT cells with p values at right.

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