Fig. 6: H3.3K27M and G34R glioma cells engraft in mouse brain and develop tumors at a higher rate than isogenic H3.3WT counterparts.

a Hematoxylin and eosin-stained images of mouse brains xenografted with our cell panel. All cell lines engrafted to at least some minimal detectable extent, but no clearly identifiable tumor cells were observed in HA samples and a tumor was present in only one of the six SF188 samples. Various clusters of tumor cells were found in the brains with all other cell lines but to a greater degree and with greater disease severity in SF-K27M and the parental XIII and XVII glioma lines. b Histological scoring of the number of tumors, estimated tumor size, tumor infiltration, and atypia severity across xenograft samples plotted as mean ± sd of scores; n = 6 mice per cell line with *p < 0.05 denoted for comparison of parental to gene-edited cell lines, Student’s t test. Scoring of individual tissue samples are in Supplementary Table 8. c, d Tumor-free survival curves of xenograft mice; n = 6 mice per cell line, *p < 0.05, log-rank test. Note: Survival curves for mice xenografted with other cell lines were not plotted as all mice survived to the maximum study duration (like the plot for HA-xenografted mice in c) and exhibited no behavioral signs of tumor despite some mice developing tumor as evidenced by the postmortem IF staining and histology.