Fig. 3: Determination of the αSyn:huPrP stoichiometry in heteroassemblies.
From: Clustering of human prion protein and α-synuclein oligomers requires the prion protein N-terminus
a Addition of unlabeled αSynO to [U-13C,15N]-labeled huPrP(23−144) results in a global signal loss in [1H,15N] HSQC NMR. For a larger version of this panel, see Supplementary Fig. 6. b Total [1H,15N] HSQC NMR signal intensity in dependence of the αSynO:huPrP(23−144) molar ratio. The solid line represents a linear fit to the data. c–e Silver-stained SDS-PAGE gels after DGC of 10 µM αSynO coincubated with either c 2 µM, d 5 µM, or e 10 µM of huPrP(23−144).
