Fig. 1: Different read-depth profiles found in L. infantum isolates from Brazil.

Del isolates contain a >12 kb deletion between 1.122 Mb and 1.135 Mb on chr31 (e.g., Del_MT_3219 in the left graph). NonDel isolates do not contain the deletion, showing full read-depth at the locus (center graph). 8HTZ isolates are heterozygous for the deletion, with read-depth dropping to ca. 50% (right graph). Quantitative PCR confirmed heterozygosity at the deletion locus in monoclonal HTZ subcultures. MIX isolates appear to contain a mixture of NonDel and Del or HTZ profiles based on subclone PCR by Carnielli et al.²⁷. However, full read-depth is observed at the deletion locus in all MIX isolates, except in MIX_PI_05A and MIX_PI_08A (showing ca. 75% read-depth, see Supplementary Fig. 4). This suggests that NonDel cells are more abundant than Del and/or HTZ cells within MIX isolates. Circle radius indicates the number of isolates (each from a different canine or human host) representing the study site. Dotted circles represent study sites where multiple read-depth profiles occur (see table inset). Fill color indicates the majority read-depth profile at such study sites. The map was created in the open-source geographic information system Quantum GIS version 2.18.4 using Open Layers plugin access to Bing Aerial imagery. Microsoft product screen shot(s) reprinted with permission from Microsoft Corporation.