Fig. 4: Characterisation of Ser-Leu interactome. | Communications Biology

Fig. 4: Characterisation of Ser-Leu interactome.

From: Global mapping of protein–metabolite interactions in Saccharomyces cerevisiae reveals that Ser-Leu dipeptide regulates phosphoglycerate kinase activity

Fig. 4

a Venn diagram showing the number of putative Ser-Leu targets identified using size exclusion chromatography (SEC), affinity purification (AP) and thermal proteome profiling (TPP) (Supplementary Data S12, S13 and S17). An overlap between at least two orthogonal approaches (86 proteins) was considered to represent the Ser-Leu interactome and is marked in black. b The Ser-Leu interactome network. Edges represent protein–protein interactions and were imported from STRING, based on the experimental evidence (score ≥ 0.4). Functionally related proteins are grouped together. Distinct colours are used to mark different protein groups. c Heatmap showing co-elution of catalytic (Ilv2) and regulatory (Ilv6) subunits of the acetolactate synthase complex with dipeptides containing branched-chain amino acids (Supplementary Data S6 and S7). The intensity was calculated relative to the maximum intensity measured across the SEC fractions. The theoretical molecular weight was calculated using reference proteins.

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