Fig. 5: Characterisation of the Pgk1–Ser-Leu interaction.
a The elution profile of Pgk1 and its putative ligand Ser-Leu (Supplementary Data S6 and S7). The intensity was calculated relative to the maximum intensity of the molecule measured across size exclusion chromatography fractions. The theoretical molecular weight (MW) was estimated using reference proteins. The Pearson correlation coefficient (PCC) indicates a correlation coefficient calculated between depicted elution profiles. b Microscale thermophoresis analysis of Pgk1 and Ser-Leu binding (Supplementary Data S26). Kd indicates dissociation constant. Data represent the means ± SD, n = 3 independent samples. c Functional validation of the interaction between Ser-Leu and Pgk1 (Supplementary Data S28). Ser-Leu significantly increases Pgk1 activity. Data represent the means ± SD, n = 3 independent samples. Asterisks denote significant difference (non-paired, two-tailed t test P value < 0.05). d Microscale thermophoresis analysis of Pgk1 and ATP binding in the presence of saturating concentrations (4 mM) of Ser-Leu (Supplementary Data S27). Kd indicates dissociation constant. Data represent the means ± SD, n = 3 independent samples.
