Fig. 1: Crystallization of the H2A.B/H2A heterotypic NCP. | Communications Biology

Fig. 1: Crystallization of the H2A.B/H2A heterotypic NCP.

From: Histone variant H2A.B-H2B dimers are spontaneously exchanged with canonical H2A-H2B in the nucleosome

Fig. 1

a Sequence alignment of H2A, H2A.B, H2A.Z, and H2A.X. The specific residues of H2A.B, H2A.Z, and H2A.X, as compared with H2A, are colored blue, orange, and green, respectively. Dashes indicate gaps in the alignment. The C-terminal region of H2A (enclosed in the red square) is replaced with that of H2A.B (enclosed in the red square), in the chimeric protein shown in Fig. 5a and Supplementary Fig. 5. (a, b) The purified NCPs were analyzed by native-PAGE with ethidium bromide staining (b) and SDS-PAGE with Coomassie Brilliant Blue (CBB) staining (c). d The crystals obtained in the presence of the H2A.B/H2A heterotypic NCP (see “Methods”). e Histones in the crystals (lane 2) and in the solution of the H2A.B/H2A heterotypic NCP (lane 3) were analyzed by SDS-PAGE with CBB staining. The uncropped gel images are shown in Supplementary Fig. 9.

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