Fig. 1: Uridine-specific endoribonuclease activity of SARS-CoV-2 Nsp15. 5′-32P-labeled RNA eicosamers were incubated at 37 °C with Nsp15. | Communications Biology

Fig. 1: Uridine-specific endoribonuclease activity of SARS-CoV-2 Nsp15. 5′-32P-labeled RNA eicosamers were incubated at 37 °C with Nsp15.

From: Tipiracil binds to uridine site and inhibits Nsp15 endoribonuclease NendoU from SARS-CoV-2

Fig. 1

Reaction products were separated in an 20% polyacrylamide gel containing 7 M urea. Digestion products of (A, B) GAACU5CAUGG10ACCUU15GGCAG20 and (C) GAACA5CAAGG10ACCAA15GGCAG20, respectively. U5-, U8-, and U14/15- uridine-specific ladder. XC and BB mark the final positions of the xylene cyanole and bromophenol blue dyes. The cleavage of the eicosamer is uridine-specific and shows no sequence preference as 5′CU↓C, 5′AU↓G, 5’CU↓U, and 5′UU↓G are recognized and cut, especially at higher manganese concentration (compare Figs. 1A and 1B). Eicosamer that does not contain uridine is not cut (Fig. 1C).

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