Fig. 2: Inhibition of SARS-CoV-2 Nsp15 endoribonuclease by Tipiracil. RNA heptamers (5′AGGAAGU), labeled at their 3′ ends with 3′,5′-[5′-32P] bisphosphates to form octamers 5′ AGGAAGU32pCp, were incubated at 30 °C with Nsp15 in the presence of Tipiracil at 0, 1.85, 3.9, 7.8, 15.6, 31.25, 62.5, 125.0, and 250 μM (Lanes 1–9). | Communications Biology

Fig. 2: Inhibition of SARS-CoV-2 Nsp15 endoribonuclease by Tipiracil. RNA heptamers (5′AGGAAGU), labeled at their 3′ ends with 3′,5′-[5′-32P] bisphosphates to form octamers 5′ AGGAAGU32pCp, were incubated at 30 °C with Nsp15 in the presence of Tipiracil at 0, 1.85, 3.9, 7.8, 15.6, 31.25, 62.5, 125.0, and 250 μM (Lanes 1–9).

From: Tipiracil binds to uridine site and inhibits Nsp15 endoribonuclease NendoU from SARS-CoV-2

Fig. 2

Lane 10: minus enzyme control. Reaction products were separated in a 20% polyacrylamide gel containing 7 M urea. 32P -labeled phosphates are marked with an asterisk. Raw image of the gel is shown in Supplementary Fig. 2. The x- and y-axes are plotted on a linear scale. Points represent two independent assays (black and open circles). Raw data for Fig. 2 are included in Supplementary Data file. The continuous line represents the best fit for the Morrison equation46.

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