Fig. 2: Subcellular localization analysis of EuTPTx. | Communications Biology

Fig. 2: Subcellular localization analysis of EuTPTx.

From: Structure–function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis

Fig. 2

a Schematic representation of chimeric construct GFP-EuTPTx. The coding regions of EuTPTx were fused to the C-terminus of GFP. XYLTCT-DsRed was used as Golgi marker. CaMV35SPRO, cauliflower mosaic virus 35S promoter; NosTer nopaline synthase terminator. b Triple-color imaging by confocal laser scanning microscopy of GFP-EuTPTx-expressing BY-2 cells. Top panels, EuTPT1; middle panels, EuTPT3; bottom panels, EuTPT5. Panels from left to right: ER marker (ER-Tracker), Golgi marker (XYLTCT-DsRed), GFP-EuTPTx, and merged images of these three fluorescence signals. (Right) Magnified images of boxed areas around the Golgi/ER/nucleus area are shown. A fluorescence intensity line scan profile was generated along the white arrow in enlarged view, which goes across the Golgi/ER/nucleus area and is shown in the right column. Yellow triangles indicate merged signals of XYLTCT-DsRed and GFP-EuTPTx. Blue, ER-Traker; red, XYLTCT-DsRed; green, GFP-EuTPTx. White scale bars: 10 μm.

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