Fig. 4: Lipogenesis and very-low-density lipoproteins (VLDL) secretion are restored in Huh7-GCK+/HK2− cells. | Communications Biology

Fig. 4: Lipogenesis and very-low-density lipoproteins (VLDL) secretion are restored in Huh7-GCK+/HK2− cells.

From: A hexokinase isoenzyme switch in human liver cancer cells promotes lipogenesis and enhances innate immunity

Fig. 4

a Quantification of intracellular lipids in total cell extracts of Huh7 and Huh7-GCK+/HK2− cells (n = 3). b Lipids and ApoB secretions in supernatants of cells cultured 24 h without FCS (n = 6 for Cholesterol, n = 3 for FFA and n = 10 for TG and ApoB). c TG/ApoB molar ratio calculated from quantifications determined in b (n = 10). d Supernatants of Huh7 and Huh7-GCK+/HK2− were analyzed by ultracentrifugation on iodixanol density gradients. ApoB was quantified in each fraction by ELISA (one representative experiment). Presented data correspond to means ± SEM of indicated number of independent experiments and p values were determined by Student’s t-test.

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