Table 1 Taxa detected at Ashmore Reef.

From: Passive eDNA collection enhances aquatic biodiversity analysis

Family name	Taxon name	Passive filtration								Active filtration
Family name	Taxon name	Charged				Non-charged				Active filtration
		4 h	8 h	12 h	24 h	4 h	8 h	12 h	24 h
Acanthuridae	Acanthurus blochii							B	B	B
	Acanthurus lineatus									A
	Acanthurus triostegus		B	B	B	B	B	B	B	B
	Acanthurus xanthopterus		B	B	B	B	B		B	B
	Ctenochaetus binotatus		B		B		B	B		B
	Ctenochaetus striatus		B		B		B	B		B
	Naso annulatus									A
	Naso brachycentron							P
	Naso unicornis									A
	Zebrasoma sp.									A
	Zebrasoma scopas									A
Albulidae	Albula glossodonta					B	B	B		B
Apogonidae	Apogon sp.							B	B	B
	Jaydia sp.							B		B
Arripidae	Arripis georgianus					P
Atherinidae	Hypoatherina temminckii				B	B	B		B	B
Balistidae	Balistapus undulatus					P		P	P
	Balistidae—unknown 1					B	B	B	B	B
	Melichthys niger						B			B
	Rhinecanthus aculeatus		B		B	B	B	B	B	B
	Rhinecanthus rectangulus									A
	Rhinecanthus verrucosus								P
Belonidae	Platybelone argalus									A
	Strongylura incisa			B			B	B		B
	Tylosurus crocodilus		B			B	B	B	B	B
Blenniidae	Aspidontus taeniatus									A
	Atrosalarias holomelas									A
	Blenniella periophthalmus				B	B			B	B
	Cirripectes sp.							P
	Salarias fasciatus							B	B	B
Carangidae	Caranx ignobilis			B	B	B				B
	Carangidae—unknown 1		B					B	B	B
Chaetodontidae	Chaetodon auriga							P
	Chaetodon vagabundus						B			B
	Chaetodon sp.									A
Cirrhitidae	Paracirrhites forsteri									A
Clupeidae	Amblygaster sirm						B			B
	Spratelloides delicatulus	B	B	B	B	B	B	B	B	B
	Spratelloides gracilis								P
Congridae	Gnathophis sp.									A
Ephippidae	Platax orbicularis	P				P
	Platax teira		B	B	B		B	B	B	B
Fistulariidae	Fistularia commersonii									A
Gadidae	Micromesistius sp.			P
Gobiidae	Bryaninops sp.									A
	Eviota sp. 1									A
	Eviota sp. 2					B	B	B		B
	Exyrias sp.									A
	Gobiodon sp.									A
	Gobiidae—unknown 1									A
	Gobiidae—unknown 2									A
	Gobiidae—unknown 3						P
	Gobiidae—unknown 4									A
	Paragobiodon sp.									A
	Vanderhorstia ornatissima									A
Haemulidae	Plectorhinchus chaetodonoides					B	B	B	B	B
Hemiramphidae	Hyporhamphus sp.								B	B
Holocentridae	Myripristis botche	B				B			B	B
	Myripristis murdjan									A
	Sargocentron rubrum									A
Kuhliidae	Kuhlia sp.									A
Kyphosidae	Kyphosus sp.					B	B	B	B	B
Labridae	Anampses sp.									A
	Chlorurus sp.									A
	Choerodon schoenleinii									A
	Cirrhilabrus exquisitus									A
	Cymolutes praetextatus	B	B			B	B	B	B	B
	Epibulus sp.		P
	Gomphosus varius						P
	Halichoeres hortulanus									A
	Halichoeres melanurus									A
	Halichoeres nebulosus									A
	Halichoeres trimaculatus		B	B		B	B	B	B	B
	Hipposcarus longiceps		B				B	B		B
	Labrichthys unilineatus		B	B		B				B
	Novaculichthys taeniourus			B					B	B
	Pseudocheilinus evanidus								P
	Pseudocheilinus hexataenia								B	B
	Scarus dimidiatus									A
	Scarus niger									A
	Scarus psittacus									A
	Scarus sp.									A
	Stethojulis bandanensis							B	B	B
	Stethojulis strigiventer								B	B
	Stethojulis sp.							B	B	B
	Stethojulis trilineata					P
	Thalassoma amblycephalum					B				B
	Thalassoma lunare						B		B	B
	Thalassoma sp.						B		B	B
Lethrinidae	Lethrinus nebulosus									A
	Lethrinus obsoletus									A
	Lethrinus sp.1					B	B		B	B
	Lethrinus sp.2									A
	Lethrinus variegatus									A
	Monotaxis grandoculis									A
Lutjanidae	Aprion virescens	B				B	B	B		B
	Caesio caerulaurea							P
	Caesio xanthonotus									A
	Lutjanus bohar		B	B		B	B	B	B	B
	Lutjanus decussatus									A
	Lutjanus fulvus							P
	Lutjanus kasmira								B	B
	Lutjanus sp. 1		B	B		B	B	B	B	B
	Lutjanus sp. 2	B				B	B	B	B	B
	Pterocaesio sp.	B	B			B				B
	Pterocaesio tile									A
Monacanthidae	Cantherhines dumerilii					P	P
	Monacanthus chinensis	P
Mugilidae	Crenimugil crenilabis						P
	Ellochelon vaigiensis		P					P
	Mulloidichthys vanicolensis					B	B		B	B
	Parupeneus barberinus									A
	Parupeneus chrysopleuron					P
	Parupeneus multifasciatus									A
	Parupeneus sp.	B	B				B		B	B
	Upeneus tragula	P
Muraenidae	Echidna nebulosa						B	B		B
	Gymnothorax buroensis									A
	Gymnothorax flavimarginatus									A
	Gymnothorax sp.					B	B	B		B
Myctophidae	Diaphus watasei									A
	Myctophidae—unknown 1									A
Myliobatidae	Aetobatus ocellatus	B	B	B	B	B	B	B	B	B
Platycephalidae	Sunagocia otaitensis						P
	Plesiops sp.						B	B		B
Pomacentridae	Abudefduf sexfasciatus/vaigiensis					B	B	B		B
	Acanthochromis sp.									A
	Amblyglyphidodon curacao									A
	Chromis atripectoralis		B							B
	Chromis atripes									B
	Chromis lepidolepis						P
	Chromis ternatensis		B							B
	Chromis sp.					B		B		B
	Chromis viridis				B					B
	Chrysiptera glauca		B		B	B				B
	Chrysiptera rex									A
	Chrysiptera sp.		B				B	B	B	B
	Dascyllus aruanus							B		B
	Dascyllus reticulatus					P	P
	Dascyllus trimaculatus			P			P
	Dischistodus prosopotaenia									A
	Hemiglyphidodon plagiometopon									A
	Neopomacentrus sp.									A
	Plectroglyphidodon lacrymatus									A
	Plectroglyphidodon leucozonus					B				B
	Pomacentrus bankanensis								P
	Pomacentrus lepidogenys							B	B	B
	Pomacentrus pavo					B		B	B	B
	Pomacentrus sp.1				B			B	B	B
	Pomacentrus sp. 2									A
	Stegastes fasciolatus									A
	Stegastes nigricans				B			B		B
Pseudochromidae	Pseudochromis sp.		B			B				B
Rhynchobatidae	Rhynchobatus sp.		P			P	P	P	P
Schindleriidae	Schindleria sp.								P
Scombridae	Auxis sp.							P
	Euthynnus sp.									A
	Scomberomorus commerson									A
Scorpaenidae	Scorpaenodes guamensis								P
Serranidae	Aethaloperca rogaa							B		B
	Cephalopholis argus					B			B	B
	Cephalopholis leopardus						P
	Epinephelus sp.						B			B
	Plectropomus laevis	B	B		B			B	B	B
	Pseudogramma polyacanthus									A
	Variola louti						B	B	B	B
Soleidae	Pardachirus pavoninus							P
Synodontidae	Trachinocephalus myops								P
Tetraodontidae	Arothron mappa								B	B
	Arothron stellatus								B	B
Xiphiidae	Xiphias gladius		P						P

Taxa at Ashmore Reef detected by passive filtration treatment and submersion time (h) compared to those detected by active filtration. An A indicates taxa detected by active filtration only, P denotes those detected by passive filtration only and B indicates taxa detected by both methods.

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Table 1 Taxa detected at Ashmore Reef.

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