Fig. 3: Cardiac glycosides modulate cytokine secretions from mixed glial cultures of microglia and pericytes.
From: Cardiac glycosides target barrier inflammation of the vasculature, meninges and choroid plexus
a, b Mixed glial cultures were treated with cardiac glycosides for 24 h prior to stimulation with IL1β for 1 h ((a) representative images). Inflammatory response was assessed by NFκB nuclear translocation in PU.1 positive microglia (c, d). e–m Conditioned media from mixed glial cultures after 24 h of vehicle (white bars), digoxin (100 nM, blue bats) or lanatoside C (1 μM, red bars) pretreatment prior to stimulation with IL1β (0.05 ng mL−1) for 24 h (n = 3, case used E215, E216, SS54). Two-way ANOVA with Tukey’s multiple comparison test, mean ± SEM.***p < 0.001, **p < 0.01, *p < 0.05.
