Fig. 2: SOP-MS analysis of LCM-dissected mouse uterine tissue.
From: Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
a Number of unique peptides and protein groups identified by the MS/MS spectra only (without MBR) from three biological replicates per cell type (luminal epithelial and stroma) and three blanks. The LCM tissue size: 100 µm (length) × 100 µm (width) × 10 µm (thickness). Each LCM-dissected tissue sample is close to ~20 cells. b Pairwise correlation of log10-transformed protein LFQ intensities between any two replicates. Pearson correlation coefficients were color coded as shown on the scale at the bottom. c Unsupervised PCA analysis based on label-free quantification of proteins expressed in luminal epithelial and stroma cells. d Volcano plot of proteins differentially expressed between the two cell types from three biological replicates per cell type.
