Fig. 2: Cytotoxicity and interaction of HDACis and TLR7as in ovarian carcinoma cells.

a The cytotoxicity of the HDACis vorinostat and romidepsin in SKOV3^WT and OvCa236 primary ovarian carcinoma cells was determined using the MTT proliferation assay after 72 h of drug exposure. Vorinostat and romidepsin are very cytotoxic in ovarian carcinoma cells (IC₅₀ ~ 4 nM). b The TLR7as imiquimod and vesatolimod were tested in the same manner as the HDACis proved less cytotoxic in these carcinoma cell types, with IC₅₀ values between 23 and 32 µM. These values were employed as reference in all subsequent biochemical experiments. The graphs are representative of n = 5 experiments. c HDACi/TLR7a interaction was analysed using isobolographic methods in SKOV3^WT and OvCa236 primary ovarian carcinoma cells isolated from ascites. The combination of HDACis and TLR7as produces a synergistic effect regardless of pairing (green arrows). Results are representative of n = 5 experiments.