Fig. 3: Results of high-pressure RCB cultivations of prioritized methanogens in the simultaneous bioreactor system (SBRS) at 10 bar.
From: Hyperthermophilic methanogenic archaea act as high-pressure CH4 cell factories
All experiments were performed in quadruplicates in the SBRS system at a gassing ratio of H2/CO2 (4:1)35. Mean and standard deviation are shown. The principle of the cultivation was to repressurize each of the bioreactors to 10 bar after full headspace gas conversion. RCB1, RCB2, RCB3, and RCB4 indicate results from individual and successive closed batch headspace gas conversions. MER / mmol L−1 h−1 is shown. The left block indicates mesophilic methanogens grown at 37 °C (Methanococcus maripaludis S2, Methanobacterium palustre F, Methanobacterium subterraneum A8p); the middle block shows the thermophilic methanogens grown at 65 °C (Methanothermobacter marburgensis Marburg, Methanobacterium thermaggregans, Methanothermobacter thermautotrophicus DeltaH, Methanothermococcus okinawensis IH1); and the right block shows hyperthermophilic methanogens (Methanocaldococcus jannaschii JAL-1 (80 °C), Methanocaldococcus vulcanius M7 (80 °C), Methanocaldococcus villosus KIN24-T80 (80 °C), Methanocaldococcus villosus KIN24-T80*-grown on 282c 18_E medium (80 °C), Methanotorris igneus Kol 5 (85 °C), Methanothermus fervidus H9 (80 °C), Methanopyrus kandleri AV19 (98 °C)).
