Fig. 3: siRNA PMP22-SQ NPs inhibit Pmp22 protein expression and promote myelination and axonal regeneration factors. | Communications Biology

Fig. 3: siRNA PMP22-SQ NPs inhibit Pmp22 protein expression and promote myelination and axonal regeneration factors.

From: Squalenoyl siRNA PMP22 nanoparticles are effective in treating mouse models of Charcot-Marie-Tooth disease type 1 A

Fig. 3

a, b Representative western blot showing the Pmp22 protein content of the different JP18 and JP18/JY13 treatment groups. Each well represents a different mouse. The protein quantification of Pmp22 was performed on at least five different mice per group for both strains. Pmp22 protein was normalized to tubulin as a reference protein. c, d Quantification analysis of immunohistochemistry images showing SOX10, Krox20, and Neurofilament on JP18 and JP18/JY13, respectively. SOX10 and Krox20 were normalized over DAPI expression to calculate their expression in percentage. Three different mice were analyzed per group. Data represent means ± s.e.m. Blue asterisks indicate significant differences between the WT and the other groups, purple asterisks indicate significant differences between 5% dextrose and the other groups, green asterisks indicate the differences between siRNA Ct-SQ NPs and the other groups and red asterisks the difference between siRNA-SQ NPs and the other groups. *p < 0.05; **p < 0.01, ***p < 0.001 using ANOVA analysis followed by Tukey’s multiple comparison tests.

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