Fig. 4: siRNA PMP22-SQ NPs are efficient to activate myelination. | Communications Biology

Fig. 4: siRNA PMP22-SQ NPs are efficient to activate myelination.

From: Squalenoyl siRNA PMP22 nanoparticles are effective in treating mouse models of Charcot-Marie-Tooth disease type 1 A

Fig. 4

a Representative TEM images of ultrathin sections of sciatic nerves of WT (n = 5) and JP18 treated mice (n = 5) (×5000 magnification) followed by g-ratio analysis of myelinated fibers. Scale bar 2 µm. The bars represent the mean. b High magnification TEM images (×120 k) show myelin layer distance (Scale bar 50 nm) and its corresponding analysis. Data represent predicted means with 95% confidence intervals for layer distance analysis. The blue lines mark a zone of 14 myelin layers for the WT and show the difference between the JP18 groups. c Same analysis was performed for JP18/JY13 mice (n = 5 per group). siRNA PMP22-SQ NPs significantly decreased the g-ratio and d the inter myelin distance in JP18/JY13 mice. In JP18/JY13 mice, a compaction of the myelin layers distance was observed by the siRNA PMP22-SQ NPs and became identical to WT. The blue lines mark a zone of 14 myelin layers for the WT and show the difference between the JP18/JY13 groups. *p < 0.05; **p < 0.01; ***p < 0.001 using ANOVA analysis followed by Tukey’s multiple comparisons test.

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