Fig. 2: Effect of seaweed diet on non-responder phenotype.

The table in the center shows the number of non-responder and responder mice in each diet group. A Design of the diet experiment. White circles denote 16S samples and are filled with the number of biological replicates for each sampling point. Black circles denote RNA-seq samples. B Percentage of mitochondria and chloroplast sequences in 16S amplicon data relative to antibiotic treatment. Colors: red—controls not treated with antibiotics, green—non-responders, blue—responders. C qPCR biomass estimates (1/Ct) for samples across response groups during antibiotics exposure. N = 12, 4, and 12 for controls, non-responders, and responders, respectively. Stars denote significance under a Mann–Whitney U test: ***p < 0.001, **p < 0.01. D Relative phyla abundances across diet and response groups. Each barchart denotes means from at least two samples and white insets are the sample size used for each barchart. Only phyla with a relative abundance larger than 0.1% are shown. Colors: red—controls not treated with antibiotics, green—non-responders, blue—responders. E PCoA of 16S samples after diet (n = 60 samples with more than 5000 reads, day ≥ 20). Symbol fill denotes sampling time relative to antibiotic treatment and colors denotes response type. Ellipses denote 95% confidence interval from a Student’s t-distribution. Dashed ellipse describes samples taken before antibiotic exposure. ASV abundances were rarefied to 5000 reads for each sample, and four samples with fewer than 5000 reads were not included in the analysis. Percentages in brackets show explained variance by that axis.