Fig. 1: Identification of Dmrt2 as a downstream transcription factor of Sox5/6/9.
From: Dmrt2 promotes transition of endochondral bone formation by linking Sox9 and Runx2
a Schematic model of RNA-seq analysis using P0 newborn mouse rib chondrocytes with adenovirus-mediated overexpression of Sox5, Sox6, and Sox9. b Expression patterns of differentially expressed genes (DEGs). c MA plot analysis of DEGs. d Classification analysis of upregulated genes with GO molecular function. Selected molecular functions from Supplemental Data 1 are shown. e–g Total RNA was isolated from primary chondrocytes (e), limb bud mesenchyme from E12.5 mouse limb buds (f), and C3H10T1/2 cells (g) infected with control (Cont), Sox9 (Sox9), or Sox5 + Sox6 + Sox9 (Sox5/6/9) adenoviruses. Col2a1 and Dmrt2 mRNA expression levels were analyzed by RT-qPCR. The RNA level is indicated as the fold increase compared with that of the control. Data are shown as the mean ± s.d. (n = 3). **p < 0.01; one-way ANOVA followed by Tukey’s multiple comparison test.
