Fig. 5: Delayed onset of chondrocyte hypertrophy in Dmrt2−/− mice.
From: Dmrt2 promotes transition of endochondral bone formation by linking Sox9 and Runx2
a, b Sections of tibiae from E14.5 (a) and E15.0 (b) WT and Dmrt2−/− littermate embryos were examined by hematoxylin and eosin (H&E) staining and RNA in situ hybridization analysis using antisense probes against Col2a1, Ihh, and Col10a1. Scale bars: 200 μm (E14.5), 500 μm (E15.0). c Quantitative analysis of Col2a1-positive resting and proliferating length, Ihh-positive length, and Col10a1-positive length in E15.0 WT and Dmrt2−/− mice. Double-headed arrows indicate the measured length in the representative images. Data are shown as the mean ± s.d. (WT, n = 6; Dmrt2−/−, n = 5). *p = 0.0023 (Ihh-positive length), p = 0.0149 (Col10a1-positive length) (vs. WT); unpaired Student’s t test.
