Fig. 1: Expression of exogenous connexins in embryonic chick lens in situ.
From: Development of a potent embryonic chick lens model for studying congenital cataracts in vivo
A At 68 h of embryonic development (around developmental stage 18), eggshells were incised to reveal live embryos (a and b). Approximately 20 nL solution containing high-titer retrovirus expressing recombinant proteins was microinjected into the lens lumens (c). B 48 h after retroviral infection, chick embryonic lenses were collected and crude membrane extracts were prepared. The expression of Cx50 and the Cx50E48K mutation was detected by western blotting using an anti-FLAG tag antibody. C 48 h after infection, chick embryonic lenses were collected and immunostained with anti-FLAG antibody and counter-nuclei stained by DAPI. White arrows show epithelial cells at the anterior region of the lens. Bar, 50 µm.
