Fig. 5: Location of Cx50E48K expression at the anterior lens region defines the severity of lens opacities. | Communications Biology

Fig. 5: Location of Cx50E48K expression at the anterior lens region defines the severity of lens opacities.

From: Development of a potent embryonic chick lens model for studying congenital cataracts in vivo

Fig. 5

A Lysates of E14 chick lenses injected with a retrovirus containing vector or Cx50E48K (Cx50E48K_clones 01 and 02, representing lenses forming mild and severe cataracts, respectively) were immunoblotted with anti-FLAG or anti-β-actin antibody. B Paraffin tissue sections of E14 chick lenses were immunostained with anti-FLAG or anti-β-actin antibody, and images were captured by light transmission (left panels) and fluorescence (right panels) microscopy. Scale bar for optical images, 400 µm, and for fluorescence images, 50 µm.

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