Fig. 7: Human crystalline mutations alter the light transmission of the lens.
From: Development of a potent embryonic chick lens model for studying congenital cataracts in vivo
Embryonic chick lenses were injected with recombinant RCAS(A) expressing WT human αA-crystalline, hCRYAA, and two mutations, hCRYAAR12C, and hCRYAAR64C. Lenses were then isolated at E14 and E20. A Lens lysates were prepared and immunoblotted with anti-FLAG or anti-β-actin antibody. Scale bar: 400 µm. B Paraffin sections of E14 chick lenses were prepared and immunolabeled with anti-FLAG and counterstained with DAPI. Scale bar: 400 µm. C Dissected whole E20 chick lenses expressing exogenous WT hCRYAA or one of two mutations were imaged with a dissecting microscope with a black (upper panel) or grid background. D The midsagittal paraffin sections of various E20 chick lenses were stained by H&E. Images were captured at a lower resolution for the entire lens and higher resolution for various regions of the lens. Scale bar: 50 µm.
