Fig. 1: Cryo-EM structure of the RSV cleaved synaptic complex (CSC) trapped with MK-2048.
From: Cryo-EM structure of the Rous sarcoma virus octameric cleaved synaptic complex intasome
a Cartoon representation of RSV CSC viewed down its pseudo twofold axis, with the 8 IN and viral DNA molecules colored differently. The two MK-2048 molecules in the active site of the catalytic IN protomers are shown in the space-filling model. Disordered CCD-CTD linkers for the distal IN dimers are shown as dashed lines. The two proximal IN dimers (green/cyan and pink/gray) plus the distal IN CTDs (magenta, slate blue) bridging between them constitute the core of the complex, referred to as ‘conserved intasome core (CIC)’. b Cryo-EM density in the same color scheme and orientation as in (a). The densities for MK-2048 are indicated and highlighted in yellow. c Side view after a 90-degree rotation about a horizontal axis from (b). The densities for distal IN CCDs in the foreground are omitted for clarity. d Model representation corresponding to the view in (c).
