Fig. 5: Single-round RSV infectivity assays.

a Sequence alignment of the RSV/HIV-1 IN tail region. The RSV IN terminal residue for each truncation is marked in red. The bottom HIV-1 IN residues are marked by percent of infectivity²⁸ and replication²⁹ of each truncation compared to wt virus. b Equivalent quantities of RSV virions produced by the indicated pRIAS-Luc constructs were used to infect DF-1 cells. The length of wt IN is 286 residues. C-terminal truncated IN mutants are indicated by the last residue in the construct. IN 1–323 has the natural 37 amino acid extension that is cleaved off in virions. Results (average ± standard deviation) compile data from five independent infection assays, each conducted with duplicate samples; data points show results of all technical replicates. c Infectivities of C-terminal IN truncations (1–264 and 1–266) and four missense IN mutants (R263A; R263K; S262P; S262R) versus pRIAS-Luc (IN 1–323) are shown (average ± standard deviation for two independent experiments, each conducted with technical duplicates). Sham samples were media harvested from DF-1 cells cultured under identical conditions except plasmids were omitted from transfection mixes. Statistical significance between samples was assessed using one-way ANOVA in GraphPad Prism version 8. ***p < 0.0001; ****p < 0.00001.