Fig. 6: Effects of LDB3 p.Ala165Val mutation on PKCα and TSC2-mTOR expression in skeletal muscle of Ldb3Ala165Val/+ mice.
Heatmap of protein levels in the vastus muscle lysates of 4 months (a) and 8-month-old (b) Ldb3Ala165Val/+ mice and their gender-matched Ldb3+/+ littermates (n = 5 per group; total 20 mice) detected by RPPA. Differentially expressed proteins with statistically significant fold changes are shown on the vertical axis (≥1.5-fold, corrected p ≤ 0.05). The levels of each protein are presented with colors, with blue for lowest and red for highest. See Supplementary Table 2. Only PKCα and TSC2 are known to localize at skeletal muscle Z-disc. c, d Immunoblotting analysis (blot and dot plot) of PKCα and TSC2 protein levels relative to vinculin in the vastus muscle of 4-month-old Ldb3Ala165Val/+ mice (n = 5 and 4, respectively) and Ldb3+/+ littermates (n = 4 and 3, respectively). e, f Immunoblotting analysis (blot and dot plot) for protein levels of PKCα and TSC2 relative to vinculin in the vastus muscle of 8-month-old Ldb3Ala165Val/+ mice (n = 4 and 5, respectively) and Ldb3+/+ littermates (n = 3 and 4, respectively). The error bars in dot plots are Mean (SEM) and represent triplicate assays. The two-tailed unpaired t-test comparison significant p values are shown. **p < 0.01; ***p < 0.001; ****p < 0.0001.
