Fig. 4: Direction of global miRNA expression changes in the thalamus and serum EVs of prion M1000 infected mice at week 3, 13 and terminal stage compared to controls.

A ANOVA analysis was performed on the normalised reads from both datasets (thalamus and serum EVs) and were plotted as Log2 fold change. miRNA found to display inverse expression in serum EVs and thalamus are indicated in red. Those found to display the same direction in expression (either upregulated or downregulated) in both sample types are indicated in green. miRNA found unchanged in both samples types are indicated in grey and those changed in one sample type are indicated in black. Thalamus prion M1000 infected week 3 (n = 4), week 13 (n = 4) and terminal (n = 5). Thalamus uninfected week 3 (n = 5), week 13 (n = 5) and terminal (n = 5). Serum prion M1000 infected week 3 (n = 6), week 13 (n = 5) and terminal (n = 5). Serum uninfected week 3 (n = 6), week 13 (n = 6) and terminal (n = 6). B miRNAs indicated in red and green from panels in (A) are displayed in a heat map to visualise the abundancy of their expression in the thamalus and serum EVs. Only miRNAs with a minimum of 5 RPM across all samples are shown here. Log₂ Mean RPM of each miRNA was used for the heat map.