Fig. 2: Flow cytometric analyses of adiponectin-expressing thymocytes.
From: Adiponectin-expressing Treg facilitate T lymphocyte development in thymic nurse cell complexes
a Tissue cryosections were prepared from thymus of ROSAmT/mG and Adn-Cre/ROSAmT/mG mice to visualize the EGFP+ cells exhibiting bright green fluorescence. b Single-cell suspensions were prepared from the thymus of Adn-Cre/ROSAmT/mG mice. Flow cytometry was performed to analyze EGFP+ cells and their surface expression of markers including CD45, CD326, CD4, CD8, and CD3. c Cell suspensions were prepared from the thymus of Adn-Cre/ROSAmT/mG mice (top). TNC complexes were then enriched by sedimentation in FBS (middle). The EGFP+ cells were visualized by real-time live confocal microscopy (bottom) [more details in Supplementary Movie 1]. d Flow cytometry was performed to analyze adiponectin-expressing EGFP+ cells in TNC complexes, and their surface expression of CD4, CD8, CD117, CD25, and Foxp3.
