Fig. 4: Primer pair clonal dominance, overlap and relative diversity, and V-region distribution of heavy and light chain in the final library. | Communications Biology

Fig. 4: Primer pair clonal dominance, overlap and relative diversity, and V-region distribution of heavy and light chain in the final library.

From: A single donor is sufficient to produce a highly functional in vitro antibody library

Fig. 4

a Cross-family comparison of clonotype dominance by individual primer sets. b Relative overlap of the HCDR3 among IGHV1–5 primer sets. c HCDR3 clonal dominance, d Cumulative clonal rank of HCDR3 assessing % representation by different copies of individual clones (e.g., 1 singletons, 2 duplicates). e LCDR3 cumulative clonal rank of the (IGKV or IGLV) LCDR3. f LCDR3 clonal dominance of the IGKV or IGLV library. Scaffold representation and Tiller’s developability criteria analysis for the f IGHV domains, g IGKV domains, and h IGLV domains.

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