Fig. 6: Phage and yeast selection against four therapeutically relevant targets shows highly developable candidates and biased biophysical profiles.
From: A single donor is sufficient to produce a highly functional in vitro antibody library
a Limited cross-reactivity of yeast scFv across the different panel of antigens at 200 nM. b Yeast scFv-display selection against a wide range 2–200 nM of antigen, shows minimal background (0 nM) with a noticeable population obtained from each selection campaign. c, d Pairwise Levenshtein distances distribution of the merged HCDR1–3 and HCDR3 amino acids. e, f HCDR3 and merged HCDR1–3 amino acid net charge tabulated using pH = 7 on Lehninger pK scale, showing the clear disparity of binders. g, h Gravy hydrophobicity index analysis of merged HCDR1–3 and HCDR3 amino acids. i HCDR3 length distribution across different antigens. j–m Analysis of the selected scaffold and their developability profile.
