Table 1 IGHV domain developability and primer sequences.
From: A single donor is sufficient to produce a highly functional in vitro antibody library
ID | Desirable | Common in natural | Neutral | Poorly developable | Primer sequence |
|---|---|---|---|---|---|
IGHV1a | 1–46, 1–69, 1–18 | 1–08 | 1–02 | CAGGTKCAGCTGGTGCAG | |
IGHV1b | 1–03 | CAGGTCCAGCTTGTGCAG | |||
IGHV1c | 1–24, 1–69–2 | SAGGTCCAGCTGGTACAG | |||
IGHV1d | 1–45, 1–58 | CARATGCAGCTGGTGCAG | |||
IGHV2a | 2–05 | CAGATCACCTTGAAGGAG | |||
IGHV2b | 2–26, 2–70 | CAGGTCACCTTGARGGAG | |||
IGHV3a | 3–07, 3–15, 3–21, 3–48, 3–53, 3–74 | 3–09, 3–64 | 3–13, 3–20, 3–43, 3–49, 3–66, 3–72, 3–38–3 | 3–73 | GARGTGCAGCTGGTGGAG |
IGHV3b | 3–11, 3–30, 3–33 | 3–30–3, 3–30–5 | CAGGTGCAGCTGGTGGAG | ||
IGHV3c | 3–23 | GAGGTGCAGCTGTTGGAG | |||
IGHV4 | 4–04 | 4–59, 4–61, 4–38–2, 4–34 | 4–28, 4–30–1, 4–30–2, 4–30–4 | 4–31, 4–39 | CAGSTGCAGCTGCAGGAG |
IGHV5a | 5–10–1, 5–51 | GARGTGCAGCTGGTGCAG | |||
IGHV6a | 6–01 | CAGGTACAGCTGCAGCAG |
