Fig. 1: High resolution video platform for tracing flies’ locomotion activities.

a Schematic of the experimental device. The cold light source was used to provide periodic light to the fruit fly to maintain the normal circadian clock; the infrared camera was placed directly above the observation area; two rows of infrared lights were arranged on both sides of this area; the food was placed at one end of the chamber and a sponge at the other. b The videos obtained by the infrared camera were analyzed by computer program (see “Methods”). c Imitating the DAMS with the trajectory of the fly. The midpoint of the y-coordinate is used to calculate how many times the fly has interrupted the infrared beam if it is in the DAMS. d–e Average daily activity profiles of male and female flies in 3 days (72 h) within each 30 min bin. The white and black columns indicate activities during the light and dark phases, respectively. f Average sleep profiles of male and female flies in 3 days within each 30 min bin. The white and black bars above indicate the light and dark phases, respectively. The blue curve is for male flies, red curve is for female flies. The error bars in (d–f) indicate SEM for five biologically independent experiments and n for the number of flies used.