Fig. 2: Dynamics of wild-type and mutant β3_GFP integrin-positive adhesions.

a, h Quantification of the β3 integrins flow in adhesions, expressed as mean displacement over time (µm/min) per replicate. Statistical analysis is provided in Supplementary Dataset 2. b Quantification of the actin flow in adhesions, expressed as mean displacement over time (µm/min) per replicate. Statistical analysis is provided in Supplementary Dataset 2. c Schematic representation of the BiFC assay used to evaluate paxillin recruitment to β3 integrins. d, e BiFC upon co-expression of mCherry_paxillin_CN and β3^WT_CC or β3^VE/YA_CC in NIH-3T3 fibroblasts. d Quantification of the BiFC efficiency. Statistical analysis is provided in Supplementary Dataset 3. e Representative TIRF images. Brightness and contrast were adjusted equally among different conditions. f, g Kymographs representative of the displacement of β3^VE/YA integrin-containing adhesions, over a time period of 10 min, in the absence (f) and in the presence (g) of mechanically (BiFC) coupled paxillin. ns (not significant) p > 0.05; *p ≤ 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Sample size, complete statistical analysis, and p values are provided in tables in supplementary datasets.