Fig. 4: Analysis of paxillin and vinculin binding upon isolation of FA-complexes. | Communications Biology

Fig. 4: Analysis of paxillin and vinculin binding upon isolation of FA-complexes.

From: Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions

Fig. 4

a, c, e Quantification of cytoplasmic proteins retained in β3WT_GFP- or β3VE_GFP-positive FAs, isolated from NIH-3T3 fibroblasts. a mCherry_paxillin, c endogenous paxillin, and e endogenous vinculin. Statistical analysis is provided in Supplementary Dataset 1. b, d, f Representative TIRF images of FAs isolated from NIH-3T3 cells of experiments quantified in a, c, and e. g, h Representative TIRF images of intact cells and FAs isolated from NIH-3T3 cells double transfected with g β3N305T+VE/YA_GFP and mCherry_paxillin and h β3VE/YA_CN and mCherry_paxillin_CC. ns (not significant) p > 0.05; *p ≤ 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Sample size, complete statistical analysis, and p values are provided in tables in supplementary datasets.

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