Fig. 10: Chicken enteroids as a model for host–protozoal interactions.
From: Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
a Brightfield image of sporozoites (closed arrow) entering caecal enteroid at 1 dpi. b–f Representative z-axis projections of chicken caecal enteroids whole-mount stained to detect cell nuclei (DAPI, blue), F-actin-expressing brush border (red) after incubation with PKH-67 labelled E. tenella (green). b Sporozoite at 2 dpi within enteroid epithelial cell and c migrating through basement membrane into lamina propria. d E. tenella trophozoite-like structures. e–f Schizogeny within enteroid epithelial cell at 9 dpi. g Schematic diagram of E. tenella lifecycle, source: impextraco.com. Scale bar: a–d, f 20 µm, e 10 µm. Images are representative of data from 2 independent experiments each with 2–3 technical replicates containing >3 embryos.
