Fig. 2: Reverse polarisation of avian floating enteroids.
From: Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
Confocal images of whole-mount enteroids stained to detect F-actin-expressing brush borders (red) and DAPI to visualise cell nuclei (blue). a Floating embryonic chicken enteroids at 2 days of culture showing epithelial cells polarised with the apical brush border (closed arrow) facing the media and basal lamina (open arrow) sits on a central core of cells. b Embryonic chicken enteroids at 7 days of culture showing ‘inside-out’ polarisation. c Tissue isolated from embryonic chicken intestine to form enteroids are villi. d Embryonic enteroids cultured in Matrigel for 2 days with epithelial cells polarised so the apical brush border is facing a central lumen. e Embryonic enteroids cultured in Matrigel for 7 days. f Isolated crypts from 9 week old chicken intestine. g Enteroids from 9 week old chickens mimic embryonic chicken enteroid polarisation at 2 days and h 7 days in floating culture. i Matrigel-embedded enteroids from 9 week old chickens at 2 days and j 7 days. k Enteroids derived from 1 week old quail also show ‘inside-out’ polarisation at 2 days and l 7 days of culture. m Isolated crypts from adult mouse intestine. n Matrigel-embedded mouse enteroid with internal lumen at 2 days in culture. o Floating mouse enteroid at 2 days in culture showing dissociated crypt cells. Scale bar: 20 µm. Images a–e, and f–o are representative of 3 cultures composed of 3 chicken embryos and 1 mouse per culture respectively.
