Fig. 7: Chicken enteroid propagation.
From: Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
a Brightfield images of representative enteroid cultures supplemented with EGF, R-spondin and Noggin at day 1 and b day 9 compared to FOM-only at c day 1 and d day 9 of culture. Chicken enteroids at e day 4 culture, f immediately post-passage and g day 3 post-passage in plain and h growth factor supplemented media. Cryopreserved crypts at i point of thaw and j after 4 days of culture compared to k freshly isolated crypts then l cultured for 4 days. Images a–i are representative of at least 3 independent cultures each containing 2–3 embryos. Scale bar: 200 µm.
