Fig. 2: The effect of LRRN4CL over-expression on metastatic colonisation.
a The number of metastatic colonies in the lungs of mice 10 days after being tail vein dosed with melanoma cell lines (5 × 105 HCmel12 cells, 4 × 105 YUMM1.7 cells and 0.25 × 105 B16-BL6 cells) that have been stably transfected with a vector carrying mouse Lrrn4cl cDNA (LRN) or an empty vector (PB). b The number of metastatic colonies in the lungs of mice 10 days after being tail vein dosed with a colorectal cell line (4 × 105 MC-38 cells), bladder cancer cell line (4 × 105 MB-49 cells), or breast cancer cell line (4 × 105 EO771 cells) that have been stably transfected with a vector carrying mouse Lrrn4cl cDNA (LRN) or an empty vector (PB). c The number of metastatic colonies in the lungs of mice 30 days after being tail vein dosed with human melanoma cell lines (2 × 105 A375 cells or 0.5 × 105 MeWo cells) or 10 days after being dosed with a mouse melanoma cell line (4 × 105 B16-F0 cells) that have been stably transfected a vector carrying human LRRN4CL cDNA (LRN) or an empty vector (PB). For a–c, each symbol represents a mouse, the bars represent mean ± SD, two independent experiments were performed (representative data from one experiment is shown) and statistics were performed using a Mann–Whitney t test. d The number of metastatic colonies in the livers of mice after being tail vein dosed (with 5 × 105 HCmel12 cells (wild-type mice) or 1 × 105 B16-F0 cells (NOD-SCID mice)) or intrasplenic dosed (0.1 × 105 B16-F0 cells (wildtype mice)) with melanoma cells that have been stably transfected a vector carrying mouse Lrrn4cl cDNA (LRN) or an empty vector (PB). Each symbol represents a mouse, the bars represent mean ± SD, and statistics were performed using a Mann–Whitney t test.
