Fig. 6: Inter-pulse evoked activity shows adaptation only with high-frequency stimulation.

a The representative aligned raw data with respect to the stimulus pulse for 130, 160, 180, and 20 Hz stimulation (0.5 ms pre-onset, up to 8 ms is shown). b The ECA delay comparison in the 0–2, 8–10, and after 22 s demonstrate the differences in adaptation between 130, 160, 180 (n = 9), and 20 Hz (n = 13) stimulations. The ECA delay, which denotes the delay of the first evoked peak, was consistent throughout the 20 Hz stimulation period, indicating no adaptation. The fastest adaptation was with 130 Hz, settling after 2 s. On each box in the boxplots, the central mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The whiskers extend to the most extreme data points not considered outliers, and the outliers are plotted individually using the “+” symbol. The individual data points are also plotted as red circles. ~ denotes p value <0.09, ** denotes significance <0.01.